Sampling Protocols

SanAir’s culture analyses protocols include a variety of agar plates specifically suited to meet client requirements.

 

Malt Extract Agar (MEA) one plate, or two plates for additional accuracy, for the isolation of environmental fungi while inhibiting the growth of bacteria. Plates are incubated at room temperature (~26°C)

Blood Agar (BAP) (Tryptic Soy Agar + 5% sheep blood) for the isolation of environmental and human commensal bacteria. (Incubated at 35°C)

MacConkey Agar (MAC) for the isolation of environmental, human commensal and coliform gram negative bacteria. (Incubated at 35°C)

Tryptic Soy Agar (TSA) for the isolation of thermophilic bacteria. (Incubated at 55°C)

The Viable Air Sampling Protocol utilizes several methods based on Andersen-style impaction sampling as well as Via-Cell cassettes. Sampling procedures should include a field blank and representative outdoor sample(s) for comparison with indoor samples. Multiple samples per site are recommended for representative results. Sampling protocols selected should best represent the individual needs of the client. A five to ten day turnaround time for cultured biological specimens is standard to provide accurate identifications and results.

•  Air Plate Sampling Protocol 139.75 Kb
•  Via-Cell^TM Sampling Protocol 101.28 Kb

Bulk samples such as insulation, wallboard, textiles and carpeting are analyzed at SanAir by serial dilution culture methods. They can be tested for fungi and bacteria.

•  Bulk Sampling Protocol

Swab surface samples are analyzed by serial dilution culture methods. Swab samples can be tested for fungi and bacteria. Swabs and area templates are provided by SanAir.

•  Swab Sampling Protocol

Air Cassette Sampling

SanAir analyzes non-viable impaction style cassettes by direct light microscopy methods.

•  Air-O-Cell^TM Sampling Protocol 102.76 Kb
•  Allergenco D^TM Sampling Protocol 105.24 Kb
•  Micro5^TM and Mold SNAP^TM Sampling Protocol 101.97 Kb
•  M2 Multimold^TM Sampling Protocol 95.37 Kb
•  Cyclex-d^TM Sampling Protocol 95.23 Kb
•  Via-Cell^TM Sampling Protocol 101.28 Kb

Surface Direct Sampling

Adhesive tape samples and bulk material can be tested for the presence of fungi by direct microscopy. Fungi can be identified to the genus level. Clear tape or a Bio-Tape^TM is necessary for tape lift sampling.

•  Bio-Tape^TM Sampling Protocol 66.43 Kb
•  Clear Tape Sampling Protocol 66.01 Kb
•  Bulk Sampling Protocol 122.25 Kb

Via-Cell^TM, Air-O-Cell^TM, MoldSNAP^TM and  Bio-Tape^TM are registered trademarks of Zefon International. For more information contact Zefon at http://www.zefon.com/

M2 Multi-Mold^TM is a registered trademark ofAllegro Industries. For more information contact Allegro Industries at http://www.allegrosafety.com/

Micro-5^TM, Cyclex-d^TM and Allergenco D^TM are registered trademarks of Environmental Monitoring Systems. For more information contact EMS at http://www.emssales.net/