SanAir’s culture analyses protocols include a variety of agar plates specifically suited to meet client requirements.
Malt Extract Agar (MEA) one plate, or two plates for additional accuracy, for the isolation of environmental fungi while inhibiting the growth of bacteria. Plates are incubated at room temperature (~26°C)
Blood Agar (BAP) (Tryptic Soy Agar + 5% sheep blood) for the isolation of environmental and human commensal bacteria. (Incubated at 35°C)
MacConkey Agar (MAC) for the isolation of environmental, human commensal and coliform gram negative bacteria. (Incubated at 35°C)
Tryptic Soy Agar (TSA) for the isolation of thermophilic bacteria. (Incubated at 55°C)
The Viable Air Sampling Protocol utilizes several methods based on Andersen-style impaction sampling as well as Via-Cell cassettes. Sampling procedures should include a field blank and representative outdoor sample(s) for comparison with indoor samples. Multiple samples per site are recommended for representative results. Sampling protocols selected should best represent the individual needs of the client. A five to ten day turnaround time for cultured biological specimens is standard to provide accurate identifications and results.
Bulk samples such as insulation, wallboard, textiles and carpeting are analyzed at SanAir by serial dilution culture methods. They can be tested for fungi and bacteria.
Swab surface samples are analyzed by serial dilution culture methods. Swab samples can be tested for fungi and bacteria. Swabs and area templates are provided by SanAir.
Air Cassette Sampling
SanAir analyzes non-viable impaction style cassettes by direct light microscopy methods.
- Air-O-CellTM Sampling Protocol 102.76 Kb
- Allergenco DTM Sampling Protocol 105.24 Kb
- Micro5TM and Mold SNAPTM Sampling Protocol 101.97 Kb
- M2 MultimoldTM Sampling Protocol 95.37 Kb
- Cyclex-dTM Sampling Protocol 95.23 Kb
- Via-CellTM Sampling Protocol 101.28 Kb
Surface Direct Sampling
Adhesive tape samples and bulk material can be tested for the presence of fungi by direct microscopy. Fungi can be identified to the genus level. Clear tape or a Bio-TapeTM is necessary for tape lift sampling.
- Bio-TapeTM Sampling Protocol 66.43 Kb
- Clear Tape Sampling Protocol 66.01 Kb
- Bulk Sampling Protocol 122.25 Kb
Via-CellTM, Air-O-CellTM, MoldSNAPTM and Bio-TapeTM are registered trademarks of Zefon International. For more information contact Zefon at http://www.zefon.com/
M2 Multi-MoldTM is a registered trademark ofAllegro Industries. For more information contact Allegro Industries at http://www.allegrosafety.com/
Micro-5TM, Cyclex-dTM and Allergenco DTM are registered trademarks of Environmental Monitoring Systems. For more information contact EMS at http://www.emssales.net/